Practical Biochemistry
331 Pages
English

Practical Biochemistry

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Description

This book discusses “Carbohydrate”, It’s a very general term that applies to a very large number of materials covering a wide spectrum of chemical structure and biological function, Carbohydrates are found in all living cells in plant, animals and microorganism. Carbohydrate may be defined as polyhydroxy aldehydes or ketones or substances that yield one of these compounds on hydrolysis.

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Published by
Published 01 January 2012
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EAN13 9796500037509
Language English
Document size 9 MB

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PracticalBiochemistry
Biochemistry Prof.Dr.AliA.JuwaiedBiochemistryAsst.Prof.Dr.AhmedA.AlAmiery Lect.AriegA.Mohammed OrganicChemistryPharmacology2012
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 سﺮﻬﻔﻟا ﺖﺳﺮﻬﻔﻟا CHAPTER – 1 CARBOHYDRATES General Reaction of Carbohydrates IIntroduction: Monosaccharide Disaccharide Oligosaccharides Polysaccharides Effect of acids on carbohydrates Molisch's Test Anthrone's Test Bial's Orcinol Test Seliwanoff's Test Effect of concentrated HNO3 solution Mucic acid test Effect of acids on carbohydrates Effect of Alkaloid on carbohydrate Benedict's, Fehling's and Barfoed's Tests Benedict's Test Fehling's test Barfoed's test Iodine test Effect of alkali on carbohydrates General Reaction of Carbohydrates II IntroductionExperiment procedure
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Hydrolysis of Sucrose Hydrolysis of Starch Fermentation of Carbohydrates Phenyl hydrazine Reaction (The formation of Osazones) Identification of Unknown Carbohydrates CHAPTER – 2 PROTEINES Color Reaction of Proteins Introduction Biuret Test Ninhydrin Test Millon's and Hopkins-Cole Test Millon's Test Hopkins-Cole Test Precipitation of Proteins Introduction Precipitation ofproteins byalkaloid reagents Precipitation of proteins by ethanol Precipitation of protein by high concentrations of neutral salts Determination of optimum conditions for precipitation ofproteins Precipitation ofprotein bysalts of heavymetals Precipitation of proteins by alkaloid reagents Precipitation of proteins by ethanol Precipitation of protein by high concentrations of neutral salts Determination of optimum conditions for precipitation ofproteins CHAPTER - 3 Lipids
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Introduction Solubilityof fats and oils Acrolein Test Saponification Properties of soap Salting out of soap Formation of fatty acid Formation of insoluble soapIodine test for fattyacids CHAPTER - 4 ENZYMES Introduction Optimize Conditions to Protect Enzyme Activity Disruption of cells /preparation of extract Precipitationpurification steps Experiment (1) Protein Determination Experiment(2)Extraction ofproteins and enzymes Experiment(3) Precipitation of protein by organic solvents Enzyme kinetics Bioassayofperoxidases Experiment(4)Determination of POD activityCatalase Bioassay Experiment (5) Determination of catalase activityExperiment (6) Kinetics of catalase Experiment (7) Determination of glucose dehydrogenase activity The effect of various factors on enzyme action Experiment (8) Effect of Temperature Experiment(9)Effect ofpH on Amylase Activity
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Experiment (10) Effect of Substrate on Amylase Activity Experiment(11)Effect of Inhibitors Nucleotides and nucleic acids Experiment(1): Isolation of RNA Experiment(2): Isolation of DNA Experiment (3): Determination of phosphorus in nucleic acids Experiment(4): RNA Estimation Experiment (5): DNA Estimation Experiment (6): estimation DNA and RNA in tissue Experiment (7): base composition of nucleic acids Experiment(8): correlation ofTm and base composition Experiment (9): effect of temperature on viscosity of DNA Experiment (10): separation of nucleic acids by MAK column
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CHAPTER ‐ ၹ CARBOHYDRATES
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