153 Pages
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A novel method for quantitative and structural determination of molecular complexes by photon antibunching [Elektronische Ressource] / vorgelegt von Haisen Ta

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153 Pages
English

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In memory of my grandmotherAcknowledgementI owe everything to all the following people, who helped me on both my research work anddaily life during my stay in Heidelberg. I can not imagine my life without their help andsupport.I am very much grateful to my advisor, PD Dr. D.-P. Herten. Since I arrived at Heidelberg,his encouragement led me into this amazing field, his guidance enlightened the wayof my research work and his support made me through all the difficulties during mystudy and research. His thoughtfulness made me feel very comfortable in my work andenjoy my life in a foreign country. Without him, it is not possible for me to come to this stage.I thank Prof. Dr. J. Wolfrum and Prof. Dr. B. Bukau for giving me the chance to study inHeidelberg University.I also thank Dr. A. Kiel, who helped me with the experiments and optical microscopes. Andthe discussions with him made me get into this field much easier and with more profoundunderstandings.I thank all the group members, Dr. K. Lymperopoulos, D. Barzan, J. Balbo, C. Spassova,T. Ehrhard, A. Kurz, A. Seefeld, Dr. K.-T. Han, Dr. P. Heinlein and so on. They gave mesuggestions on research and helped me, who is a foreigner, with my daily life in Germany.Especially I thank Dr. C. Roth, who introduced Labview to me and gave me a lot of helpon programming. I thank Dr. D. Siegberg, who helped me on the photostabilizing buffer. Ialso thank M.

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Published 01 January 2010
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Language English
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In memory of my grandmother
Acknowledgement
I owe everything to all the following people, who helped me on both my research work and daily life during my stay in Heidelberg. I can not imagine my life without their help and support.
I am very much grateful to my advisor, PD Dr. D.-P. Herten. Since I arrived at Heidelberg, his encouragement led me into this amazing field, his guidance enlightened the way of my research work and his support made me through all the difficulties during my study and research. His thoughtfulness made me feel very comfortable in my work and enjoy my life in a foreign country. Without him, it is not possible for me to come to this stage.
I thank Prof. Dr. J. Wolfrum and Prof. Dr. B. Bukau for giving me the chance to study in Heidelberg University.
I also thank Dr. A. Kiel, who helped me with the experiments and optical microscopes. And the discussions with him made me get into this field much easier and with more profound understandings.
I thank all the group members, Dr. K. Lymperopoulos, D. Barzan, J. Balbo, C. Spassova, T. Ehrhard, A. Kurz, A. Seefeld, Dr. K.-T. Han, Dr. P. Heinlein and so on. They gave me suggestions on research and helped me, who is a foreigner, with my daily life in Germany. Especially I thank Dr. C. Roth, who introduced Labview to me and gave me a lot of help on programming. I thank Dr. D. Siegberg, who helped me on the photostabilizing buffer. I also thank M. Schwering, who helped me on the labeling of DNA and also gave me many suggestions on my thesis. I thank A. Rybina and T. Lisauskas for their support during my thesis writing. I also thank Dr. Katharina Stoehr, who was the one I should have shared more time with.
I would like to thank Prof. Dr. M. Mayer, Dr. A. Mogk, Dr. G. Kramer, Dr. T. Haslberge and A. Sandikci for their collabrations during my Ph.D work.
I also thank Y. Wang for her proofreading of my thesis and support during my thesis writing.
I also thank the financial support of the Heinz Goetze Memorial Fellowship Program and Dr. D. Wuensche for her nice organization of the scholarship. I thank the financial support from Stipendien- und Betreuungsprogramm (STIBET) from DAAD during my dissertation preparation.
I owe a lot to my parents and my sister. Their unconditional love and support make me through all the difficulties and make me happy all the time no matter how far I am.
There are many many people having helped me in my Ph.D work, I can not list everyone here. Their help have benefited and will benefit me in my future research work and life.
Abbreviation
AOM APD APS BSA CCD CW DNA FIDA FIFO FLIM FRET FWHM HF HPLC IC IRF LEDs LM NA PCH
Acousto-Optic Modulator
Avalanche Photodiodes
amino-propyl silane
bovine serum albumin
Charge-Coupled Device
Continuous Wave
deoxyribonucleic acid
Fluorescence Intensity Distribution Analysis
First-In-First-Out Fluorescence Lifetime Imaging Fluorescence Resonance Energy Transfer Full Width at Half Maximum hydrofluoric acid
High Performance Liquid Chromatography
Internal Conversion
Instrument Response Function
Light Emitting Diode
Levenberg-Marquardt
Numerical Aperture
Photon Counting Histogram
PLL PMT ROXS SMD SMFS SMS SPC TCSPC TIRF TTTR VR
poly-l lysine
Photomultiplier Tube
Reducing-Oxidizing System
Single Molecule Detection
Single-Molecule
Fluorescence
Single Molecule Spectroscopy
Single Photon Counting
Spectroscopy
Time-Correlated Single Photon Counting
Total Internal Reflection Fluorescence Microscopy
Time-Tagged Time-Resolved
Vibrational Relaxation