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Aggregation of hybrid molecules and film formation of colloidal monolayers [Elektronische Ressource] / Ting Liu

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Aggregation of Hybrid Molecules and Film Formation of Colloidal Monolayers Dissertation zur Erlangung des Grades "Doktor der Naturwissenschaften" am Fachbereich Chemie, Pharmazie und Geowissenschaften der Johannes Gutenberg-Universität Mainz Ting Liu geboren in Heilongjiang (V. R. China) Mainz – 2011 Dekan: 1. Berichterstatter: 2. Berichterstatter: Datum der mündlichen Prüfung: 08. Dezember 2011 D77 For my parents 我我我我的的的的父父父父母母母母 “I was born not knowing and have had only a little time to change that here and there.” Richard Feynman Contents Contents Contents ........................................................................................................................ I 1 Introduction and Motivation ................................................................................ 1 1.1 DNA Hybrid Materials .................................................................................. 4 1.2 Polymeric Colloidal Monolayer .................................................................... 8 2 Theory and Methods .......................................................................................... 13 2.1 Fluorescence Correlation Spectroscopy ...................................................... 13 2.1.1 Fluorescence Process ...........................................................................

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Published 01 January 2012
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Language English
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Aggregation of Hybrid Molecules and Film
Formation of Colloidal Monolayers


Dissertation
zur Erlangung des Grades
"Doktor der Naturwissenschaften"

am Fachbereich Chemie, Pharmazie und Geowissenschaften
der Johannes Gutenberg-Universität Mainz



Ting Liu
geboren in Heilongjiang (V. R. China)

Mainz – 2011


















Dekan:
1. Berichterstatter:
2. Berichterstatter:

Datum der mündlichen Prüfung: 08. Dezember 2011


D77




For my parents
我我我我的的的的父父父父母母母母














“I was born not knowing and have had only a little time to change that here and
there.”
Richard Feynman




Contents

Contents

Contents ........................................................................................................................ I
1 Introduction and Motivation ................................................................................ 1
1.1 DNA Hybrid Materials .................................................................................. 4
1.2 Polymeric Colloidal Monolayer .................................................................... 8
2 Theory and Methods .......................................................................................... 13
2.1 Fluorescence Correlation Spectroscopy ...................................................... 13
2.1.1 Fluorescence Process ........................................................................... 15
2.1.2 Fluorescence Correlation Spectroscopy ............................................... 16
2.1.3 Fluorescence Cross-Correlation Spectroscopy .................................... 21
2.2 Micromechanical Cantilever ....................................................................... 24
2.2.1 Dynamic Mode .................................................................................... 25
2.2.2 Static Mode .......................................................................................... 27
3 FCS Study of DNA Hybrid Micelles ................................................................. 31
3.1 Calibration of Observation Volume with Rh6G ......................................... 37
3.2 Diffusion Coefficient of Reference DNAs .................................................. 39
3.3 Diffusion Coefficient of dsDNA88mer-4PDI ............................................. 42
3.4 Design of Concentrated Solution of dsDNA88mer-4PDI ........................... 44
I Contents

3.5 Diffusion Coefficient of Concentrated Reference Sample .......................... 45
3.6 Diffusion Coefficient of Concentrated dsDNA88mer-4PDI ....................... 48
3.7 Summary ...................................................................................................... 51
4 FCCS Study of Size and Residence Time of DNA-b-PPOs ............................... 53
4.1 Estimation of Micelle Size ........................................................................... 56
4.2 Estimation of Residence Time of dsDNA-b-PPO ....................................... 58
4.3 Summary ...................................................................................................... 61
5 Coating Cantilever with Colloidal Monolayer ................................................... 63
5.1 Coating Cantilever with Colloidal Monolayer ............................................. 66
5.2 Adjust Particle Distance by Plasma Etching ................................................ 71
5.3 Coating Cantilevers at the End .................................................................... 72
6 Film Formation of Colloidal Monolayers on a Micromechanical Cantilever .... 75
6.1 Dynamic Mode ............................................................................................ 76
6.1.1 Mass Loading of PS Colloidal Monolayer ........................................... 76
6.1.2 Thermomechancial Properties of PS Colloidal Monolayers ................ 77
6.1.3 Calculation of Young’s Modulus .......................................................... 85
6.1.4 Thermal Annealing of Cross-linked PS Particles ................................. 87
6.1.5 Estimation of Young’s Modulus of Crosslinked PS Films ................... 90
6.1.6 Film formation via Organic Vapor Annealing ..................................... 90
6.1.7 Toluene Absorption of PS Colloids ...................................................... 93
6.2 Static Mode .................................................................................................. 96
6.2.1 Non crosslinked PS Colloidal Monolayers ........................................... 97
II Contents

6.2.2 Crosslinked PS Colloidal Monolayers ............................................... 102
6.2.3 Variation of Toluene Vapor Activity ................................................. 106
6.3 Summary ................................................................................................... 109
7 Polymeric Functionalized Cantilevers as Glucose Detectors .......................... 112
7.1 Mass of Coating Layer .............................................................................. 116
7.2 Topological Analysis ................................................................................ 116
7.3 Surface Stress Change upon Glucose Binding .......................................... 118
7.4 Height Response of Polymer Brushes to Glucose ..................................... 121
7.5 Summary ................................................................................................... 123
8 Experimental .................................................................................................... 124
8.1 FCS Investigation of Shape of DNA Hybrid Aggregates ......................... 124
8.1.1 Materials ............................................................................................ 124
8.1.2 Hybridization of ssDNA .................................................................... 124
8.1.3 FCS Measurements ............................................................................ 125
8.2 FCCS Investigation of the Residence Time of Unimers ........................... 125
8.3 Coating Cantilever with Colloids .............................................................. 126
8.3.1 PS Particles ........................................................................................ 126
8.3.2 Plasma Treatment of Colloids ............................................................ 126
8.4 Study of Film Formation of Colloidal Monolayer by Cantilever ............. 127
8.4.1 Micromechanical Cantilever Sensors ................................................ 127
8.4.2 Deflection Measurement .................................................................... 127
8.4.3 Resonance Frequency Measurement ................................................. 128
III Contents

8.4.4 Mass Flow Controller ......................................................................... 129
8.4.5 Dynamic Mechanical Analysis ........................................................... 131
8.5 Glucose Detection by Polymeric Functionalized Cantilever ..................... 131
8.5.1 Polymer Brush Synthesis .................................................................... 131
8.5.2 Cantilever Arrangement ..................................................................... 132
8.5.3 Resonance Frequency Measurement of Cantilever ............................ 132
8.5.4 Confocal Profilometer ........................................................................ 132
8.5.5 Scanning Force Microscopy ............................................................... 132
8.5.6 Deflection Measurement of Cantilever............................................... 133
9 Summary and Conclusion ................................................................................. 134
9.1 Aggregation Analysis by FCS ................................................................... 134
9.2 Mechanical Analysis of Polymer Films by Cantilever .............................. 135
9.3 Application of Polymeric Functionalized Cantilevers as Sensors ............. 137
10 Reference .......................................................................................................... 140
List of Publications ................................................................................................... 148
Conference Contribution .......................................................................................... 148
List of Abbreviations ................................................................................................ 149

IV