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Correlation between the functional impairment of bone marrow-derived circulating progenitor cells and the extend of coronary artery disease

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Bone marrow-derived circulating progenitor cells (BM-CPCs) in patients with coronary heart disease are impaired with respect to number and functional activity. However, the relation between the functional activity of BM-CPCs and the number of diseased coronary arteries is yet not known. We analyzed the influence of the number of diseased coronary arteries on the functional activity of BM-CPCs in peripheral blood (PB) in patients with ischemic heart disease (IHD). Methods The functional activity of BM-CPCs was measured by migration assay and colony forming unit in 120 patients with coronary 1 vessel (IHD1, n = 40), coronary 2 vessel (IHD2, n = 40), coronary 3 vessel disease (IHD3, n = 40) and in a control group of healthy subjects (n = 40). There was no significant difference of the total number of cardiovascular risk factors between IHD groups, beside diabetes mellitus (DM), which was significantly higher in IHD3 group compared to IHD2 and IHD1. Results The colony-forming capacity (CFU-E: p < 0.001, CFU-GM: p < 0.001) and migratory response to stromal cell-derived factor 1 (SDF-1: p < 0.001) as well as vascular endothelial growth factor (VEGF: p < 0001) of BM-CPCs were reduced in the group of patients with IHD compared to control group. The functional activity of BM-CPCs was significantly impaired in patients with IHD3 as compared to IHD1 (VEGF: p < 0.01, SDF-1: p < 0.001; CFU-E: p < 0.001, CFU-GM: p < 0.001) and to IHD2 (VEGF: p = 0.003, SDF-1: p = 0.003; CFU-E: p = 0.001, CFU-GM: p = 0.001). No significant differences were observed in functional activity of BM-CPCs between patients with IHD2 and IHD1 (VEGF: p = 0.8, SDF-1: p = 0.9; CFU-E: p = 0.1, CFU-GM: p = 0.1). Interestingly, the levels of haemoglobin AIc (HbAIc) correlated inversely with the functional activity of BM-CPCs (VEGF: p < 0.001, r = −0.8 SDF-1: p < 0.001, r = −0.8; CFU-E: p = 0.001, r = −0.7, CFU-GM: p = 0.001, r = −0.6) in IHD patients with DM. Conclusions The functional activity of BM-CPCs in PB is .

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Published 01 January 2012
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BozdagTuranet al. Journal of Translational Medicine2012,10:143 http://www.translationalmedicine.com/content/10/1/143
R E S E A R C HOpen Access Correlation between the functional impairment of bone marrowderived circulating progenitor cells and the extend of coronary artery disease 1*1*1 11 1 Ilkay BozdagTuran, R Goekmen Turan, Liliya Paranskaya , Nicole S Arsoy , C Hakan Turan , Ibrahim Akin , 1 11 11 12 Stephan Kische , Jasmin Ortak , H Schneider , S Ludovicy , Tina Hermann , Giuseppe DAncona , Serkan Durdu , 2 11 A Ruchan Akar , Hueseyin Inceand Christoph A Nienaber
Abstract Background:Bone marrowderived circulating progenitor cells (BMCPCs) in patients with coronary heart disease are impaired with respect to number and functional activity. However, the relation between the functional activity of BMCPCs and the number of diseased coronary arteries is yet not known. We analyzed the influence of the number of diseased coronary arteries on the functional activity of BMCPCs in peripheral blood (PB) in patients with ischemic heart disease (IHD). Methods:The functional activity of BMCPCs was measured by migration assay and colony forming unit in 120 patients with coronary 1 vessel (IHD1, n= 40),coronary 2 vessel (IHD2, n= 40),coronary 3 vessel disease (IHD3, n = 40)and in a control group of healthy subjects (n= 40).There was no significant difference of the total number of cardiovascular risk factors between IHD groups, beside diabetes mellitus (DM), which was significantly higher in IHD3 group compared to IHD2 and IHD1. Results:The colonyforming capacity (CFUE: p<0.001, CFUGM: p<0.001) and migratory response to stromal cell derived factor 1 (SDF1: p<0.001) as well as vascular endothelial growth factor (VEGF: p<0001) of BMCPCs were reduced in the group of patients with IHD compared to control group. The functional activity of BMCPCs was significantly impaired in patients with IHD3 as compared to IHD1 (VEGF: p<0.01, SDF1: p<0.001; CFUE: p<0.001, CFUGM: p<= 0.003,0.001) and to IHD2 (VEGF: p= 0.001,CFUE: p= 0.003;SDF1: pNo= 0.001).CFUGM: p significant differences were observed in functional activity of BMCPCs between patients with IHD2 and IHD1 (VEGF: p = 0.8,SDF1: p= 0.9;CFUE: p= 0.1,CFUGM: p= 0.1).Interestingly, the levels of haemoglobin AIc (HbAIc) correlated inversely with the functional activity of BMCPCs (VEGF: p<0.001, r=0.8 SDF1: p<=0.001, r0.8; CFU E: p= 0.001,r =0.7, CFUGM: p= 0.001,r =0.6) in IHD patients with DM. Conclusions:The functional activity of BMCPCs in PB is impaired in patients with IHD. This impairment increases with the number of diseased coronary arteries. Moreover, the regenerative capacity of BMCPCs in ischemic tissue further declines in IHD patients with DM. Furthermore, monitoring the level of BMCPCs in PB may provide new insights in patients with IHD. Keywords:Circulating progenitor cells, Migration capacity, Colony forming capacity, Ischemic heart disease, Diabetes, Coronary artery disease
* Correspondence: ilkayboztur@googlemail.com; dr_g_turan@hotmail.com Equal contributors 1 Department of Internal Medicine, Division of Cardiology, University hospital Rostock, Ernst Heydemann Str 6, Rostock 18055, Germany Full list of author information is available at the end of the article
© 2012 BozdagTuran et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.