178 Pages
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DNA hybrid materials consisting of oligonucleotides and organic molecules [Elektronische Ressource] : synthesis, characterization and applications / Diana Milena Anaya

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‘DNA Hybrid Materials Consisting of Oligonucleotides and Organic Molecules: Synthesis, Characterization and Applications’ Dissertation zur Erlangung des Grades “Doktor der Naturwissenchaften” am Fachbereich Chemie, Pharmazie und Geowissenschaften der Johannes Gutenberg-Universität Mainz Diana Milena Anaya geb. in Bogotá, Kolumbien Mainz 2010 1 Dekan: 1. Berichterstatter: 2. Berichterstatter: Tag der mündlichen Prüfung: 2 3 4 Dedicated to my parents 5 6 “Science knows no country, because knowledge belongs to humanity, and is the torch which illuminates the world.” (Louis Pasteur ) 7 8 9 TABLE OF CONTENTS LIST OF ABBREVIATIONS ...............................................................................13 Chapter 1 INTRODUCTION ................................................................................................

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Published 01 January 2010
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‘DNA Hybrid Materials Consisting of
Oligonucleotides and Organic Molecules:
Synthesis, Characterization and Applications’







Dissertation
zur Erlangung des Grades
“Doktor der Naturwissenchaften”




am Fachbereich
Chemie, Pharmazie und Geowissenschaften
der Johannes Gutenberg-Universität Mainz











Diana Milena Anaya
geb. in Bogotá, Kolumbien
Mainz 2010


1
Dekan:
1. Berichterstatter:
2. Berichterstatter:
Tag der mündlichen Prüfung:



































2












































3







































4












Dedicated to my parents























5







































6




















“Science knows no country, because knowledge
belongs to humanity, and is the torch which
illuminates the world.”
(Louis Pasteur )













7







































8




9 TABLE OF CONTENTS


LIST OF ABBREVIATIONS ...............................................................................13

Chapter 1

INTRODUCTION ................................................................................................17

1.1 DNA fundamentals.......................................................................................18
1.2 DNA as a construction material for nanoarchitectures .................................25
1.3 DNA-based hybrid materials ........................................................................29
1.4 Summary......................................................................................................32
1.5 References...................................................................................................34
Chapter 2

MOTIVATION AND OBJECTIVES.....................................................................39

Chapter 3

AGGREGATION OF DOUBLE STRANDED DNA INDUCED BY AROMATIC
π-SYSTEMS.......................................................................................................43

3.1 Introduction ..................................................................................................43
3.2 Preparation of dsDNA-PDI hybrids ..............................................................44
3.3 Analysis on surface by Atomic Force Microscopy (AFM) .............................46
3.4 Native polyacrylamide gel electrophoresis (PAGE) Analysis .......................48
3.5 Fluorescence correlation spectroscopy (FCS) study of DNA-PDI hybrids in
.solution .........................................................................................................49
3.6 Photophysical study of DNA-PDI hybrids.....................................................52
3.7 Conclusions .................................................................................................55
3.8 Experimental Section ...................................................................................57
3.9 References...................................................................................................66
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