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Genetic code engineering with methionine analogs for synthetic biotechnology [Elektronische Ressource] / Carlos Guillermo Acevedo Rocha

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194 Pages


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Published 01 January 2010
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Language English
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Max-Planck-Institut für Biochemie

Genetic code engineering with methionine analogs for synthetic biotechnology

Carlos Guillermo Acevedo Rocha

Vollständiger Abdruck der von der Fakultät für Chemie der Technischen Universität
München zur Erlangung des akademischen Grades eines

Doktors der Naturwissenschaften

genehmigten Dissertation.

Vorsitzender: Univ.-Prof. Dr. Michael Groll
Prüfer der Dissertation:
1. Univ.-Prof. Dr. Nediljko Budisa
2. Univ.-Prof. Dr. Thomas Kiefhaber

Die Dissertation wurde am 24.06.2010 bei der Technischen Universität München
eingereicht und durch die Fakultät für Chemie am 23.07.2010 angenommen.

To my lovely family

“To put the world right in order, we must first put the nation in order; to put the nation
in order, we must first put the family in order; to put the family in order, we must first
cultivate our personal life; we must first set our hearts right.”


Parts of this work are planned to be, or have been submitted to publication:

Nehring S*, Acevedo-Rocha CG*, Hoesl MG*, et al. Lipase congeners with
enhanced resistance toward additives. (*contributed equally; in preparation)

Hoesl MG*, Acevedo-Rocha CG*, Nehring S*, et al. Lipase congeners designed by
genetic code engineering. (*contributed equally; submitted)

Poster presentations:

Acevedo-Rocha CG, Merkel L, and Budisa N. “Translation initiation by means of β-
Cyclopropylalanine” 2008. International Conference on aminoacyl-tRNA synthetases:
From basic mechanisms to systems biology, Veyrier-du-Lac, France

Other scientific publications:

Acevedo-Rocha CG, et al. Synthetic cells and genomes (in preparation)

Acevedo-Rocha CG, Geiermann AS, Budisa N, Merkel L. In vivo unnatural
translation initiation. (in preparation)

Sharp L and Acevedo-Rocha CG. New Frontiers in Science. Offspring (Official
magazine from the Max Planck PhDnet; in press)

Acevedo-Rocha CG, Yu J y Dobay A. Entgrenzung – transcending boundaries
across scientific disciplines. Arkhaï 2010; (14)23-40

Acevedo-Rocha CG and Steinbach J. The Art of Science and the Science of Art.
Offspring 2009; (4)8-13

Acknowledgments │ I


I would like to thank very much the following people:

Prof. Dr. Nediljko Budisa for his kindness, openness, support, motivation,
originality, good sense of humour, priceless freedom, exciting discussions about
science, history, politics, and the world. He provided me a solid ground where I
could grow as a person and as an open-minded scientist.

Prof. Dr. Michael Groll and Prof. Dr. Thomas Kiefhaber for allowing me to
present them my dissertation.

Prof. Dr. Thomas Kiefhaber, Prof. Dr. Elena Conti, Dr. Daniel Wilson, and Dr.
Birgit Wiltschi for being part of my thesis advisory committee.

Prof. Dr. Dieter Oesterhelt and his incredible friendly group of people for
permitting me to work in his department of “Membrane Biochemistry”.

Prof. Dr. Garabed Antranikian and Dr. Maryna Royter for allowing me to
cooperate and to learn new things about the exciting field of biocatalysis.

Prof. Dr. Zoya Ignatova for her motivation and input with my thesis work, and for
the original scientific cooperations.

Prof. Dr. Yves Mechulam and Prof. Dr. Peter Schultz for sending kindly some of
the plasmids used in this work.

Prof. Dr. Robert Huber for giving me the great chance to meet his student.

Dr. Caroline Koehrer for her technical help and nice scientific discussions.Acknowledgments │ II

Dr. Harald Huber, Prof. Dr. Nick Talbot, Dr. Michael Kershaw, Dr. Gaia
Travosanis, Mrs. Tatjana Kleine, Mr. Christian Hoffman, and Mrs. Sylvia
Reddehase for giving me genomic DNA or cDNA from different organisms.

Dr. Joerg Tittor, Dr. Gong Zhang, and Mr. Wolfgang Schloegl for their special
help with radioactive experiments.

Mrs. Elisabeth Weyher-Stingl, Mr. Snezan Marinkovic and Mr. Reinhard Mentele
for their great expertise and help concerning scientific issues.

Mrs. Petra Birle, Mrs Tatjana Krywcun, and Mrs. Traudl Wenger for her
invaluable technical assistance and help, especially for the cloning work and the
purification of alloproteins.

Dr. Birgit Wiltschi for her great supervision, exciting scientific discussions, and
helpful manners to make me think differently about science, as well as her
feedback with the thesis and her organized way of working.

Dr. Yuri Cheburkin for his supervision during my first internship at the Budisa
lab, and for his technical aid and advises.

Dr. Lars Merkel for his cordial support in the lab, synthesis of amino acids, great
input with my thesis, and for this great teachings in chemistry and art.

Prof. Dr. Shouliang Dong for his great support with the synthesis of amino acids.

Mr. Martin Turk for his great help and expertise related to enzyme modeling.

Mrs. Pamela David for her exact and great proof-reading quality of my thesis.