PNA-ligand bioconjugates as potential building blocks for sequence-specific, metal-mediated DNA-RNA-cleavage [Elektronische Ressource] / presented by Tim Kersebohm

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Dissertation submitted to the Combined Faculties for the Natural Sciences and for Mathematics of the Ruperto-Carola University of Heidelberg, Germany for the degree of Doctor of Natural Sciences presented by Dipl.-Chem. Tim Kersebohm born in Dortmund rdOral Examination: February 23 , 2005 PNA-Ligand Bioconjugates as Potential Building Blocks for Sequence-Specific, Metal-Mediated DNA-/RNA-Cleavage Referees: Prof. Dr. Nils Metzler-Nolte Prof. Dr. Andres Jäschke This work was carried out between December 2000 and November 2004 at the Institute of Pharmacy and Molecular Biotechnology, Department of Chemistry Ruperto-Carola University of Heidelberg, Germany Where is the Wisdom, We lost in Knowledge Where is the Knowledge, We lost in Information Where is the Information, We lost in Data T.S. Eliot (1888-1965) Acknowledgements I am very grateful to everybody who supported me during my work and life, no matter if professionally or personally. My gratitude especially concentrates on the following people: Prof. Dr. Nils Metzler-Nolte for luring me to Heidelberg and giving me the opportunity to face all challenges we had to overcome together while building up research and teaching in our department – the students will nevertheless appreciate that one day, I am sure… Prof. Dr.

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Published 01 January 2005
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Dissertation
submitted to the
Combined Faculties for the Natural Sciences and for Mathematics
of the Ruperto-Carola University of Heidelberg, Germany
for the degree of
Doctor of Natural Sciences
















presented by
Dipl.-Chem. Tim Kersebohm
born in Dortmund

rdOral Examination: February 23 , 2005





PNA-Ligand Bioconjugates as
Potential Building Blocks for Sequence-Specific,
Metal-Mediated DNA-/RNA-Cleavage




















Referees: Prof. Dr. Nils Metzler-Nolte
Prof. Dr. Andres Jäschke


This work was carried out between December 2000 and November 2004 at the
Institute of Pharmacy and Molecular Biotechnology, Department of Chemistry
Ruperto-Carola University of Heidelberg, Germany




















Where is the Wisdom, We lost in Knowledge
Where is the Knowledge, We lost in Information
Where is the Information, We lost in Data
T.S. Eliot (1888-1965) Acknowledgements
I am very grateful to everybody who supported me during my work and life, no matter
if professionally or personally.
My gratitude especially concentrates on the following people:

Prof. Dr. Nils Metzler-Nolte for luring me to Heidelberg and giving me the opportunity
to face all challenges we had to overcome together while building up research and
teaching in our department – the students will nevertheless appreciate that one day, I am
sure…

Prof. Dr. Reinhard Brossmer for his patriarchal and inspiring spirit.

Dr. Ulrich Hoffmanns for attending me for the last 10 years, being my friend and buddy
and staying solid as a rock in a world of strange adversities. Without you, dear Uli, I
wouldn’t have made it at all.

Dr. Sreçko Kirin, Thomas Happ and Andrea Maurer for the cooperation with the PNA
monomer synthesis, for checking out the solid phase and melting curve stuff, introduc-
ing these techniques to our group and for fruitful discussions about science and life.
Special thanks to Dr. Sreçko Kirin for providing me with the bpa know-how, many
creative controversies and several hints and for reviewing this thesis.

Xavier deHatten for being French.

Dina Pavlovic Rosman, Fozia Noor, Dr. Janine Chantson, Dr. Judy Caddy, Dr. Daniel
Milani for their collegiality and their support.

Richard Wombacher for introducing the terms “krasse knowledge”, “krasse Ansage”
“pang” and “difficile” and everybody else from The Jäschke Group.
Dr. Walter Kramer for technical and theoretical assistance and for “keeping the educa-
tional level”.

Heiko Rudy for being one of the best technical employees one could imagine by antici-
pating one’s every wish. Keep on like this, and everybody will value your work.

Ute Hertle and Tobias Timmermann for measuring numerous NMR spectra.
Angelika Seith, Bianca Flock and Dr. Ulrich Schatzschneider for the measurement of
MALDI-TOF and ESI spectra.

Viola Funk for her helpfulness, her trustability and her commitment in so many cases.
Karin Weiß for being a real natural wonder of cheerfulness – somebody should write a
PhD thesis about this phenomenon. Karin, I’d like you to be my personal lifetime-
assistant !

The students of Pharmacy and Molecular Biotechnology for playing the counterpart to
life in the lab and for providing new friends.

My parents for their love, security, admiration, support, respect and my spiritual and
social home.