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Response and resilience of methanotrophs to disturbances [Elektronische Ressource] / Adrian Kah Wye Ho. Betreuer: Peter Frenzel

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Response and resilience of methanotrophs to disturbances Doctoral thesis for the fulfillment of the grade of Doktor der Naturwissenschaften (Dr. rer. Nat) Submitted to the Faculty of Biology of the Philipps University of Marburg / Lahn By Adrian Ho Kah Wye from Penang, Malaysia. Marburg / Lahn 2010 Pledge I certify that this thesis entitled: “Response and resilience of methanotrophs to disturbances” was performed without any unlawful devices. I did not use any other than the described literature sources or technical devices. This work has never been submitted before in this or similar form to any other universities, and has not been used before any examination. Marburg, 01 October 2010 Adrian Ho Kah Wye. This work was carried out between October 2007 and October 2010 at the Department of Biogeochemistry, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany under the supervision of Prof. Dr. Peter Frenzel. Doctoral thesis submitted on: Date of oral examination: First reviewer: Prof. Dr. Peter Frenzel Second reviewer: Prof. Dr.

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Published 01 January 2011
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Language English
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Response and resilience of
methanotrophs to disturbances













Doctoral thesis for the fulfillment of the grade of
Doktor der Naturwissenschaften (Dr. rer. Nat)


Submitted to the Faculty of Biology of
the Philipps University of Marburg / Lahn







By Adrian Ho Kah Wye

from Penang, Malaysia.







Marburg / Lahn 2010



































Pledge

I certify that this thesis entitled:

“Response and resilience of methanotrophs to disturbances”

was performed without any unlawful devices. I did not use any other than the
described literature sources or technical devices. This work has never been
submitted before in this or similar form to any other universities, and has not
been used before any examination.

Marburg, 01 October 2010


Adrian Ho Kah Wye.


















































This work was carried out between October 2007 and October 2010 at the
Department of Biogeochemistry, Max Planck Institute for Terrestrial
Microbiology, Marburg, Germany under the supervision of Prof. Dr. Peter
Frenzel.




























Doctoral thesis submitted on:

Date of oral examination:

First reviewer: Prof. Dr. Peter Frenzel

Second reviewer: Prof. Dr. Roland Brandl









































The following papers have been published or are in preparation by the date of
submission of this thesis:


Ho, A., Lüke, C. and Frenzel, P. (2010) Recovery of methanotrophs from
disturbance: population dynamics, evenness, and functioning. ISME J.
Accepted.

Ho, A. and Frenzel, P. Response and resilience of paddy soil methanotrophs
to heat shock. In preparation.

Ho, A., Lüke, C., Bannert, A., Cao, Z.H. and Frenzel, P. Aging well:
methanotrophic potential and methanotroph along a chronosequence of 2000
years. In preparation.

Ho, A. and Frenzel, P. Methanotrophic activity and composition in young and
ancient rice paddies. In preparation.





















Table of Contents

Summary III
Zusammenfassung V

1. Introduction

1.1 Methane cycle 1
1.2 Rice paddies as anthropogenic methane sources 2
1.3 Methanotrophs 4
1.4 The pmoA gene marker 10
1.5 Methanotrophs as model microorganisms to study biodiversity
and ecosystem functioning (BEF) 11
1.6 Aims of study 14
1.7 References 16

2. Recovery of methanotrophs from disturbance: population dynamics,
evenness, and functioning.

2.1 Abstract 21
2.2 Introduction 22
2.3 Experimental procedure 24
2.4 Result 29
2.5 Discussion 37
2.6 Supplementary materials 45
2.7 References 48

3. Response and resilience of paddy soil methanotrophs to heat shock.

3.1 Abstract 51
3.2 Introduction 52
3.3 Results and Discussion 54
3.4 Supplementary materials 64
3.5 References 67
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4. Aging well: methanotrophic potential and methanotroph along a
chronosequence of 2000 years.

4.1 Abstract 69
4.2 Introduction 70
4.3 Experimental procedure 72
4. Result 76
4.5 Discussion 83
4.6 Supplementary materials 87
4.7 References 88

5. Methanotrophic activity and composition in young and ancient rice paddies.

5.1 Abstract 91
5.2 Introduction 92
5.3 Experimental procedure 94
5.4 Results and discussion 98
5.5 References 105

6. General discussion and outlook

6.1 Recovery of methanotrophs from disturbances: function 108
6.2 Recovery of methanotrophs from disturbances:
community composition 110
6.3 Diversity of pmoA gene in paddy soils 112
6.4 Comparison of culture-independent approaches 114
6.5 Summary 115
6.6 Outlook 116
6.7 References 117

Acknowledgement 119
Curriculum vitae 121

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