Groupe d’Etude et de Contrôle
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Groupe d’Etude et de Contrôle

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17 Pages
English

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Groupe d'Étude et de Contrôle des Variétés et des Semences Group for the Study and Control of Varieties and Seeds Secteur Étude des Variétés Variety Study Department Experimental Protocol Turf Grasses Value for Cultivation and Use Trials Version to be use for the 2010 season DOCVAT/GAZ/PROTO/003/IND3 10 pages, 3 annexes Principal changes: GENERAL frame p. 6 § 4: Observations results p. 8 § 5: Transfer and treatment of data Annex 2: Network trial locations GEVES Network manager Vincent Gensollen Unité expérimentale La Valette 711 rue Jean-François Breton 34090 MONTPELLIER Tél. 04 67 04 35 80 Fax 04 67 63 37 58 vincent.gensollen@geves.fr GEVES trials network Comité Technique Permanent de la Sélection des plantes cultivées DOCVAT/GAZ/PROTO/003/IND3 Summary 1. Goal of the Experimentation ......................................................................................... 1 2. General Experimental Principles .................. 1 2.1 Enrolment Contract for the CTPS GEVES Trials Network ...................................... 1 2.2 Receipt of seeds for trials ...................................................................................... 1 2.3 Variety list conformity ............................ 2 2.4 Variety identification in the trial .............. 3 2.5 Visits to trials ......................................................................................................... 3 3. ...

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Groupe d'Étude et de Contrôle
des Variétés et des Semences
Group for the Study and Control
of Varieties and Seeds

Secteur Étude des Variétés
Variety Study Department



Experimental Protocol


Turf Grasses

Value for Cultivation and Use
Trials

Version to be use for the 2010 season

DOCVAT/GAZ/PROTO/003/IND3

10 pages, 3 annexes

Principal changes:
GENERAL frame
p. 6 § 4: Observations results
p. 8 § 5: Transfer and treatment of data
Annex 2: Network trial locations

GEVES Network manager
Vincent Gensollen
Unité expérimentale La Valette
711 rue Jean-François Breton
34090 MONTPELLIER
Tél. 04 67 04 35 80
Fax 04 67 63 37 58
vincent.gensollen@geves.fr

GEVES trials network Comité Technique Permanent de la Sélection des plantes cultivées DOCVAT/GAZ/PROTO/003/IND3
Summary
1. Goal of the Experimentation ......................................................................................... 1
2. General Experimental Principles .................. 1
2.1 Enrolment Contract for the CTPS GEVES Trials Network ...................................... 1
2.2 Receipt of seeds for trials ...................................................................................... 1
2.3 Variety list conformity ............................ 2
2.4 Variety identification in the trial .............. 3
2.5 Visits to trials ......................................................................................................... 3
3. Experimental procedure 4
3.1 Trial layout - Surface of the plots ........... 4
3.2 Management of the trial ......................................................................................... 4
3.2.1 Sowing .......................................................................................................... 4
3.2.2 Method and frequency of mowing . 4
3.2.3 Fertilization ... 4
3.2.4 Weed control ................................................................................................ 5
3.2.5 Complementary protection ............ 5
3.2.6 Irrigation ....... 5
3.2.7 Wear ............................................................................................................. 5
4. Observations, results .... 6
4.1 Characters recorded .............................................................................................. 6
4.2 Rating scale and scoring ....................... 7
5. Transfer and treatment of data ..................................................................................... 8
5.1 Agronomic data ..................................... 8
5.2 Plot data ................ 8
5.3 Missing value ......................................................................... 9
5.4 Confidentiality of data ............................................................ 9
5.5 Transfer of data to GEVES .................... 9
5.6 Validation of the trials ................................ 9
6. Contacts for any additional information required ..................... 10
List of annexes ................................................................................................ 1

Annex 1: Enrolment contract for the CTPS trials network ..... 2 pages
Annex 2: Network trial locations .............. 1 page
Annex 3: List of turf grass species .......................................................................... 1 page


VCU Experimental Protocol - Turf Grasses -2010 Season DOCVAT/GAZ/PROTO/003/IND3
1. Goal of the Experimentation
The Trial network of CTPS (Permanent Technical Committee for breeding) is managed and
placed under the control of GEVES. It has for object the testing of the Value for Cultivation
and Use (VCU) of new candidate varieties presented for registration to the Official
Catalogue.
The object of the present protocol is to describe the experimental procedures used in the
framework of the trial of the Value for Cultivation and Use (VCU) for the study of the turf
grass varieties.

The aim of the trials is to evaluate the behaviour of these varieties in comparison with control
varieties selected for their “agronomic” value and sales. Every year, the control varieties are
chosen by the “Fodder crop and Turf” section of CTPS. The trials last three years.

At the end of the 3-year study, varieties whose value is judged to be satisfactory, in
comparison with the check varieties, are admitted for VCU.

The GEVES trials network comprises locations in France and in other European countries
(see the list of locations in annex 2). It gathers trials conducted at experimental stations of
Institutes for Agricultural Research or Offices for varieties registration, at private plant
breeding stations, and at agricultural schools. The species concerned are listed in annex 3.
2. General Experimental Principles
2.1 Enrolment Contract for the CTPS GEVES Trials Network

Within the CTPS experimental network, all plant material comprising the varieties under trial
supplied to trial hosts is subject to restricted use and conditions of confidentiality.

An enrolment contract is provided by GEVES which must be signed by all trial
hosts/establishments taking part in the GEVES Trials Network and is binding with respect to
the procedures stipulated therein (annex 1).

The contract remains in force for the entire period during which the trial host takes part in the
GEVES Trials Network irrespective of the species under study. In the event of a change of
name, or of a merger of the establishment concerned, the contract (annex 1) will be renewed
and signed.

NB: the terms of the enrolment contract can be included in a more general contract
which deals with other aspects. In such a case, there might be no need to sign the
model enrolment contract.

2.2 Receipt of seeds for trials

The seed management department of GEVES receives, packages and dispatches to trial
hosts the seed lots that are grouped according to trials (GEVES Magneraud, BP 52 - 17700
SURGERES France - Contact: Xavier De LAFFOREST - Phone + 33 546 68 30 89 or +33
546 68 30 26).
At each location, the trial host receives a sample in a bag labelled with the name of the
species and the variety, the number of the cultivar, and the trial series.
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The quantity of seed supplied for each variety varies with the species:
Species Name Latin Species Name Quantity
Creeping Bent Agrostis stolonifera L 100 grams
Browntop Bent Agrostis capillaris L 100 grams
Cocksfoot Dactylis glomerata L. 200 grams
150 grams (300 g
Tufted hairgrass Deschampsia caespitosa depending on the plot
surface)
Tall Fescue Festuca arundinacea Schreber 250 grams (500 grams
depending on the plot
surface)
Hard and Sheep’s Fescues Festuca ovina L. 200 grams (400 grams
depending on the plot
surface)
Slender Creeping Red Festuca rubra L. Ssp litoralis 200 grams (400 grams
Fescues Festuca rubra L. Ssp pruinosa depending on the plot
surface)
Chewing Fescue Festuca rubra L. Ssp 200 grams (400 grams
commutata depending on the plot
surface)
Strong Creeping Red Fescuca rubra L. Ssp rubra 200 grams (400 grams
Fescue depending on the plot
surface)
150 grams (300 g
Crested hairgrass Koeleria macrantha depending on the plot
surface)
Timothy Phleum bertolonii DC 150 grams
Rough-Stalked Meadow- Poa trivialis L 150 grams
Grass
Smooth-Stalked Meadow- Poa pratensis L 150 grams (300 grams
Grass depending on the plot
surface)
Perennial Ryegrass Lolium perenne L. 200 grams (400 grams
depending on the plot
surface)

A traceability form is included in the seed kits. The form must be returned to GEVES
Magneraud, if an anomaly is observed.

2.3 Variety list conformity

The lists of varieties are composed of new candidate varieties in study for VCU and of check
varieties defined annually. The lists of varieties are identical for the whole trial network
concerned.
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The lists of varieties are enclosed in the seed kits dispatched by GEVES. The lists are
validated by the VCU trials network manager (“GRES” according to the French acronym)
who will contact the trial hosts if necessary.
On reception, the trial hosts should ensure that the batches of seeds they receive
match the list or lists included in the experimental protocol.
In the case of a discrepancy, the trial host should contact the network manager
without delay.
The addition or withdrawal of any variety is subject to the approval of the network
manager, solely authorised to modify the list of the varieties in trial.
Use of varieties under trial for border plots or additional plots in the trial is prohibited.

To complete a trial or for borders, aside from the controls supplied by GEVES, the trial host
shall only use registered varieties from the European catalogue or turf mixtures sold in
Europe.
2.4 Variety identification in the trial

In no case should the labelling of the plots enable identification of the candidate varieties in
trial.

This means that labels must not mention:
The breeder reference of the candidate variety (generally code associating letters and
figures);
The cultivar or CTPS number of the candidate variety;
The name of the breeder or applicant of the candidate variety.

Conversely, it is allowed to mention any codes permitting identification within the plot, such
as iteration number, x and y coordinates... The check varieties may be identified.

2.5 Visits to trials

As CTPS trials are confidential, they can only be inspected by GEVES officials, CTPS
experts, or the Breeders (or their representatives) of the varieties under study in the trials.
In this case, the visit is limited to the examination of their candidate varieties and the
standard varieties.
The visit by any authority or person other than those listed above is strictly prohibited.
Every visit must take place in the presence of the trial host or of his/her representative.

In addition, each year a regional controller of GEVES inspects trials located in his sector by
means of one or more visits. A report of his observations and opinion on the validity of the
trials is sent to the network manager. A general discussion with the trial host enables the
regional controller to check that management and scoring procedures are being respected
and to take note of any particular problems encountered.

In order to facilitate the inspection by the regional controller, the trial host should provide
before the visit a map of access to the turf VCU trials location, and a detailed map of the trial
including the codes of the varieties under trial to be controlled.

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3. Experimental procedure
3.1 Trial layout - Surface of the plots

Trials must be carried out in 3 blocks with randomised varieties within each species (RCB) (a
trial is composed of all the varieties belonging to the same species). Each trial host should
draw his own layouts for the trials.
2 Each plot should have a surface area of at least 2 m to enable correct appraisal of the
different characters.

3.2 Management of the trial
3.2.1 Sowing
Sowing is by broadcast and the dose varies according to the species:

Species Seed rate (g/m2)
Rough-stalked and Smooth-stalked meadowgrass 15,
Hard and Sheep’s fescues 20
Red fescues 20
Perennial Ryegrass 20
Tall fescue 25
Other species Please, contact the GEVES

Depending on the type of soil at each site, up to 50% additional seed can be used to
compensate for seed emergence difficulties.
To limit the risk of pollution from one plot to another, the seeds should be sowed within a
wooden frame (which also delimits the plot) made of planks 0.30 meter wide. Seeds can be
tilled under with a rake.
Additional remark:
Partial re-sowing after emergence is not recommended even if the rate of emergence is too
low.
3.2.2 Method and frequency of mowing
The grass should be mowed for the first time when the grass in the plots showing the highest
“growth” reaches 80 to 100 mm. The cutting height is of 50 – 60 mm.
With successive mowing, the cutting height should gradually be reduced to 25 - 30 mm.
The turf is to be mowed weekly (or twice a week), at least in periods of maximum growth
(spring and the beginning of autumn).
3.2.3 Fertilization
Application of fertilizer corresponds to relatively intensive turf maintenance. Even though the
different species do not have the same needs, all the species at each site should receive the
same dose, that is to say:
Nitrogen: 150 - 160 kg / ha / year
Phosphoric acid : 70 - 80 kg / ha / year
Potash : 120 - 130 kg / ha / year
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The frequency and timing of amendments depend on the product: agricultural mineral
fertilizers and/or “slow-release” artificial organic fertilizers.


3.2.4 Weed control
Broadleaf weeds should be treated after the first or second mowing. Only approved weed
killers should be used, that are in great numbers and efficient, and the majority of which do
no damage turf grasses. In case of doubt, the trial host should not hesitate to contact the
network manager.

In the case of pollution by other grasses, these will have to be removed by hand or treated
chemically, although in the latter case certain precautions should be taken (choice of
molecule, timing and dose of the product) depending on the species. Only approved products
should be used. For more information on this subject, the trial host should contact the
network manager and/or the website of the Ministry of Agriculture for France : http://e-
phy.agriculture.gouv.fr/ or other official sites.

In order to have a better assessment method of the persistency, it is not recommended to
weed the trial the third year.
3.2.5 Complementary protection
Fungicides should not be used, as this type of treatment can hinder the evaluation of the
disease resistance of the variety.
3.2.6 Irrigation
If necessary, plots should be irrigated to ensure the establishment/survival of the plants.
However, in some trials the plots may be regularly irrigated in the second and third years,
depending on the climatic conditions.
3.2.7 Wear
Wear is simulated using a machine equipped with spikes on one part of each plot once a
week during specific periods: “winter” or “summer”, depending on the location.

Two types of equipment are used by the Network:

- Wear machine “lawn”: it compacts the soil and the aboveground parts of the turf. This
machine simulates moderate pedestrian use similar to that encountered on lawns in
public parks or in private homes, and is applied in “summer” (mid May to mid
September).

- Wear machine “sport”: The other machine has a double drums made up of two parts that
turn at different speeds, which, in addition to the compacting action described above, also
rips up the grass. This action simulates what happens on a sports ground (soccer or
rugby ground), and is used during the games period. Treatment stops in very bad
weather conditions.

To avoid wear treatment affecting the regularity of mowing, it is recommended as much as
possible to mow at reduced speed and on the diagonal using a machine that is not too
heavy. It is also possible to locate wear trials elsewhere, as is the case at the experimental
site at Les Alleuds (France). However, the proximity of the two parts of the plot (subjected or
not to wear) facilitates observations.
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4. Observations, results
After sowing, the form entitled “VCU trials: changes in the experiment” should be filled out
and sent back to the network manager (trial host’s name, town, location of the trial, number
of iterations, trial layout, effectively sown or added varieties, choice of mode of sending the
results).
Any substantial modification of the place of trial will be indicated to the network manager
before sowing.
Whether there is a modification or not of the trial host, it is necessary to note: the name,
address, phone number, and e-mail address of the trial host. The list of the trial hosts, thus
drawn and updated every year, facilitates contacts and visits of the trials.

All these particulars are very important as they allow :
- The publication of the official and definitive list of varieties;
- Corrections to the information temporarily stored and necessary for the edition of
GEVES files of results.
The edition of all the GEVES files imposes that all the “VCU trials: changes in the
experiment” forms be returned to GEVES immediately after sowing.
4.1 Characters recorded
Turf grasses are scored on the basis of visual observations only, enabling assessment of the
characters that are considered to be indispensable to judge the value for use of the varieties.

Each character must be coded using a 3-letter code which the trial host uses to collect data
and transfers to GEVES network manager.

- 1 - Establishment is judged by the amount of ground covered by the cultivar two months
(IN2) and six months (IN6) after sowing.

- 2 - Wear Tolerance is judged by estimating the abundance of the vegetation that
remains after artificial wear trials (ground cover) every month till half of the treatment
period.

- 3 - Disease Tolerance; the main diseases observed in the different species of turf
grasses are:
Rusts (Uromyces, Puccinia spp) (RMR) encountered in all species,
Drechslera diseases (Helminthosporium spp or Drechslera spp) (RMH),
encountered in all species,
Red thread (Corticium fuciforme) (RMC) encountered in all species,
Fusarium patch encountered in all species mainly due to Microdochium nivale,
Fusarium nivale (RMF) a less frequently Fusarium roseum (RMS),
Heterosporium (Heterosporium spp) in Timothy.
Other diseases that are sometimes encountered in turf include Sclérotinium or
Dollar spot (slerotinia homeocarpa) (RMD), Anthracnose (Colletotrichum spp),
Myxomycose, Curvulariose, etc. In such a case, the trial host should record the
name of the disease receiving one or more scores.

- 4 - Leaf colour (COU). It is appreciated in spring in the absence of stress and disease
by the colour of the leaves.

- 5 - Shoot Density (DEN). It is to scored the capacity of a variety to establish a dense
sward. It is appreciated in spring in the absence of stress and of disease.
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- 6 - Fineness of leaves (FIN). It is appreciated in spring in the absence of stress and
disease by the fineness of the leaves.

- 7 - Persistency (PRN) is judged at the end of the trial period by the condition of the plant
population in the part of the plots that did not receive the wear treatment.

- 8 - General aesthetic qualities (AEG) or General merit; this is the overall score for the
behaviour of the sward: it is the result of interactions between external factors (weather
conditions, parasites, nutrition), and the presence of weeds, the colour and the fineness of
the leaves, and shoot density. This score should be awarded once a month; the final
score is the average of the different scores awarded during the course of the trials.



It is compulsory to record all these characters. However, depending on the conditions
(climatic or other), it may not be possible to record some characters (for example in the case
of the absence of disease or trials too degraded to appreciate the density). In that case, it is
necessary to justify the situation to the network manager or to the GEVES regional
controllers responsible for the agronomic validation of trials.
4.2 Rating scale and scoring
Characters 1, 2, 3, 4, and 7 are scored as follows:

Note Expression
9 Corresponds to 100% of ground cover, green aspect, plant
presence, and absence of diseases and weeds.
8 as above for the following values > 87.5 % and < 100 %
7 e for the following values > 75 % and < 87.5 %
6 as above for the following values > 62.5 % and < 75 %
: 5 as above for the following values > 50 % and < 62.5 %
: 4 as above for the following values > 37.5 % and < 50 %
: 3 e for the following values > 25 % and < 37.5 %
: 2 as above for the following values > 12.5 % and < 25 %
: 1 as above for the following values > 0 % and < 12.5 %

Character 5, leaf colour is scored as follows:

Note Expression Note Expression
9 very dark green 4 medium green to light green
8 dark green to very dark green 3 light green
7 dark green 2 light green to very light green
6 medium green to dark green 1 very light green
5 medium green

Character 6, Fineness of leaves is scored as follows:
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Note Expression Note Expression
9 very narrow 4 Medium to wide
8 very narrow to narrow 3 wide
7 narrow 2 wide to very wide
6 Medium to narrow 1 very wide
5 Medium

For character 8, General aesthetic qualities, the score goes from 9 (perfect turf) to 1
(completely degraded).
5. Transfer and treatment of data
The transfer of the data is made on an IT support according to a standard defined by GEVES
(DIANE).

NB: when not using DIANE, an Excel file will be supplied to the trial host by the
network manager for the transfer of data (DIANE will be recommended everywhere once
translated into English).

The trial host shall install DIANE software (to be downloaded from GEVES web site at http:
//www.geves.fr, from the sub-web site “Accès professionnel”, in column “Produits et
prestations” on the page “Protocoles et D i an e ” or to be sent on a CD Rom). The note of
installation of the software and the user guide of "DIANE" are at the disposal of the trial host
on various supports (web site, CD Rom or paper version on demand addressed to the
network manager).
In order to always have the latest version of the software, it is recommended to update it
systematically in every new trial campaign by download (at the address indicated above), or
from the CD Rom.
GEVES supplies, for every trials realized by the trial host, a file allowing data collection in a
merged file.

The email sent by GEVES includes all the trials realized by a trial host, every trial being
differentiated by its trial code.

To each GEVES file *.(Year) A corresponds a single trial.
Two parts are to be filled: an «agronomic data» file and a «Plot data» file which lists all the
recorded characters. In both cases, the decimal separator is the period (full stop punctuation
mark).
5.1 Agronomic data
The «agronomic data» part permits to check the validity of the trial by listing all agronomic
interventions. The comment of the trial host is thus indispensable and completes the
appreciation made by the GEVES regional controllers responsible for the agronomic
validation of trials.
The merging under Diane produces a *.(Year)G file.
5.2 Plot data
The «Plot data» file stores all the characters recorded. In this file, the sheet titled
"Observation on the plot" allows, if necessary, to give a comment or a precision on every plot
of the trial.
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