The role of PGRP proteins in innate immunity pathways in the malaria vector Anopheles gambiae [Elektronische Ressource] / presented by Stephan Meister

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SUMMARY Innate immunity is the first line of defense against invading microorganisms and provides clues to adaptive immunity for the development of memory for subsequent infections. Insects, similar to other invertebrates, do not have adaptive immunity and thus rely on their innate immune system to combat infections. We have analyzed the role of the peptidoglycan (PGN) receptor protein (PGRP) family and other components of innate immune signaling pathways in the immune defense of the mosquito Anopheles gambiae, the main vector of human malaria in sub-Saharan Africa. The PGRP gene family consists of seven genes with ten PGRP domains. We have shown that from all PGRP genes only PGRPLC has a role in the resistance to bacterial infections of both Gram types. In our experiments we have used the Gram-positive bacterium Staphylococcus aureus and the Gram-negative bacterium Escherichia coli. The PGRPLC gene encodes at least three isoforms that derive from infection-driven alternative splicing of a pool of immature transcripts. Each isoform contains a different PGRP domain, encoded by three exons that all contribute equally to the PGN binding pocket. Structural modeling of the PGRPLC isoforms revealed a potential for all isoforms to bind both types of PGN, the Lys-type, which is mostly found in Gram-positive bacteria and the DAP-type, mostly found in Gram-negative bacteria.

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Published 01 January 2006
Reads 45
Language English
Document size 10 MB
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