Validation and establishment of cell culture models to study invasion and feto-maternal interaction in the bovine placentome [Elektronische Ressource] / by Bridger, Philip Simon
114 Pages
English
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Validation and establishment of cell culture models to study invasion and feto-maternal interaction in the bovine placentome [Elektronische Ressource] / by Bridger, Philip Simon

Downloading requires you to have access to the YouScribe library
Learn all about the services we offer
114 Pages
English

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VALIDATION AND ESTABLISHMENT OF CELL CULTURE MODELS TO STUDY INVASION AND FETO-MATERNAL INTERACTION IN THE BOVINE PLACENTOMEPHILIP SIMON BRIDGERINAUGURAL DISSERTATION (Cumulative Thesis)in partial fulfillment of the requirementsfor the PhD-Degree of the Faculties of édition scientifiqueVVB LAUFERSWEILER VERLAG Veterinary Medicine and Medicineof the Justus-Liebig-University GiessenISBN 3-8359-5270-6VVB LAUFERSWEILER VERLAGSTAUFENBERGRING 15D-35396 GIESSENTel: 0641-5599888 Fax: -5599890redaktion@doktorverlag.dewww.doktorverlag.de 9 7 8 3 8 3 5 9 5 2 7 0 6édition scientifiqueVVB VVB LAUFERSWEILER VERLAGPHILIP S. BRIDGER BOVINE PLACENTOMAL CELL CULTURE MODELS. Das Werk ist in allen seinen Teilen urheberrechtlich geschützt. Jede Verwertung ist ohne schriftliche Zustimmung des Autors oder des Verlages unzulässig. Das gilt insbesondere für Vervielfältigungen, Übersetzungen, Mikroverfilmungen und die Einspeicherung in und Verarbeitung durch elektronische Systeme.1. Auflage 2008All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means, electronic, mechanical, photocopying, recording, or otherwise, without the prior written permission of the Author or the Publishers.

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Published 01 January 2008
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VALIDATION AND ESTABLISHMENT OF
CELL CULTURE MODELS TO STUDY INVASION
AND FETO-MATERNAL INTERACTION
IN THE BOVINE PLACENTOME
PHILIP SIMON BRIDGER
INAUGURAL DISSERTATION
(Cumulative Thesis)
in partial fulfillment of the requirements
for the PhD-Degree of the Faculties of
édition scientifique
VVB LAUFERSWEILER VERLAG Veterinary Medicine and Medicine
of the Justus-Liebig-University GiessenISBN 3-8359-5270-6VVB LAUFERSWEILER VERLAG
STAUFENBERGRING 15
D-35396 GIESSEN
Tel: 0641-5599888 Fax: -5599890
redaktion@doktorverlag.de
www.doktorverlag.de 9 7 8 3 8 3 5 9 5 2 7 0 6
édition scientifique
VVB VVB LAUFERSWEILER VERLAG
PHILIP S. BRIDGER BOVINE PLACENTOMAL CELL CULTURE MODELS. Das Werk ist in allen seinen Teilen urheberrechtlich geschützt.
Jede Verwertung ist ohne schriftliche Zustimmung des Autors
oder des Verlages unzulässig. Das gilt insbesondere für
Vervielfältigungen, Übersetzungen, Mikroverfilmungen
und die Einspeicherung in und Verarbeitung durch
elektronische Systeme.
1. Auflage 2008
All rights reserved. No part of this publication may be
reproduced, stored in a retrieval system, or transmitted,
in any form or by any means, electronic, mechanical,
photocopying, recording, or otherwise, without the prior
written permission of the Author or the Publishers.
st
1 Edition 2008
© 2008 by VVB LAUFERSWEILER VERLAG, Giessen
Printed in Germany
VVB LAUFERSWEILER VERLAG
édition scientifique
STAUFENBERGRING 15, D-35396 GIESSEN
Tel: 0641-5599888 Fax: 0641-5599890
email: redaktion@doktorverlag.de
www.doktorverlag.deValidation and establishment of cell culture models to study
invasion and feto-maternal interaction
in the bovine placentome



Inaugural Dissertation
(Cumulative Thesis)
in partial fulfillment of the requirements
for the PhD-Degree
of the Faculties of Veterinary Medicine and Medicine
of the Justus-Liebig-University Giessen


by
Bridger, Philip Simon
of
Nairobi, Kenya



Giessen 2008

_________________________________________________________________
From the Department of Veterinary Anatomy, Histology and Embryology
Director: Prof. Dr. M. Bergmann
Faculty of Veterinary Medicine, Justus-Liebig-University Giessen


















Committee Members:
stProf. Dr. Christiane Pfarrer (1 Supervisor and Internal Thesis Examiner)
Priv. Doz. Dr. Christine Wrenzycki (External Thesis Examiner)
Prof. Dr. Dr. Hans Michael Piper (Chairman of the Oral Panel)
ndProf. Dr. Klaus Steger (2 Supervisor)





rdDate of Doctoral Defense: 3 April 2008 Declarations
_________________________________________________________________





“I declare that I have completed this dissertation single-handedly without the
unauthorized help of a second party and only with the assistance acknowledged therein.
I have appropriately acknowledged and referenced all text passages that are derived literally
from or are based on the content of published or unpublished work of others, and all
information that relates to verbal communications. I have abided by the principles of good
scientific conduct laid down in the charter of the Justus-Liebig-University of Giessen in
carrying out the investigations described in the dissertation.”

„This dissertation is submitted as a cumulative thesis according to the charter of the
PhD-Program of the Faculty of Veterinary Medicine and Medicine of the Justus-Liebig-
University. The thesis includes an interconnection of three original papers, two of which
describe and discuss the majority of my experimental work during the course of the program.
The experimental data from the third paper was produced during the same time period in
collaboration with a colleague (Susanne Haupt) who submitted part of the work as doctoral
dissertation.”




Philip Bridger

_________________________________________________________________ Dedications
_________________________________________________________________








To Nicole
_________________________________________________________________ Table of contents
_________________________________________________________________
Table of contents

1 ABBREVIATIONS ................................................................................................... 1
2 LIST OF PAPERS .................................................................................................... 2
3 INTRODUCTION 3
3.1 THE BOVINE PLACENTA ....................................................................................... 3
3.1.1 The feto-maternal interface ........................................................................ 4
3.1.2 Comparative placentation .......................................................................... 6
3.2 PLACENTA AND INVASION ................................................................................... 7
3.3 BOVINE PLACENTAL DISORDERS 8
3.3.1 The retained placenta ................................................................................. 8
3.3.2 Bovine viral diarrhea 9
3.4 CELL CULTURES OF THE PLACENTOME ............................................................... 11
3.4.1 Epithelial cell cultures from the caruncle ................................................ 11
3.4.2 Trophoblast cell cultures from the cotyledon ........................................... 13
3.5 AIM OF THE STUDY ............................................................................................ 14
4 MATERIAL AND METHODS ............................................................................. 16