Biology Scope approved at audit 2 Aug, 09
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Biology Scope approved at audit 2 Aug, 09

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NABL 2009 INTERFIELD LABORATORIES, COCHIN-682005 SCOPE OF ACCREDITATION - MICROBIOLOGY SL GROUP OF SPECIFIC TESTS OR TYPES OF SPECIFICATION, STANDARD ( RANGE OF TESTING/ MU NO. PRODUCTS, TESTS PERFORMED METHOD) OR TECHNIQUE USED LIMIT OF DETECTION (±) Antilog MATERIALS OR ITEMS TESTED 1 WATER E. coli/250ml IS 15185 : 2002 Absent/Present NA (PACKAGED Coliforms/250ml NA DRINKING WATER Faecal streptococci/250ml IS 5887 (part2): 1976 NA OTHER 0.3MPN THAN Staphylococcus Absent/Present NA NATURAL aureus/250ml MINERAL Pseudomonas IS: 13428 ANNEX D.1998 NA WATER aeruginosa /250ml IS Sulphite reducing bacteria IS:13428 ANNEX C.1998 Absent/Present NA 14543:2004 /50ml PACKAGED Aerobic microbial IS 5402 :2002 1 cfu/ml 0.0742 NATURAL 0.0299 count/ml at 22? C & 37?C MINERAL Yeast & Mould/250ml IS: 5403.1999 Absent/Present NA WATER IS Salmonella/250ml IS 15187 (part 3):1999 NA 13428:1998 Shigella/250ml IS 5887 ( part 7):1999 NA DRINKING Vibrio cholerae/250ml IS: 5887 (PART V) NA WATER Vibrio IS 5887 (part 5) :1976 Absent/Present NA IS parahaemolyticus/250ml 10500:1991 PROCESS Coliform bacteria IS 1622: 1981: reaffirmed MPN =1/100ml NA WATER in 1996 IS Standard plate count 1 cfu/ml 0.0299 4251:1967 in 1996 98/83/EC Proteolytic bacteria IS 4251: 1967 Appendix A 5 cfu/ml NA Lipolytic bacteria 5 cfu/ml NA stE. coli/100 ml APHA:21 Ed 9221C, F 1 cfu 0.044 MPN =1.8 - 1600 Page 1 of ...

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NABL 2009 INTERFIELD LABORATORIES, COCHIN-682005
SCOPE OF ACCREDITATION - MICROBIOLOGY


SL GROUP OF SPECIFIC TESTS OR TYPES OF SPECIFICATION, STANDARD ( RANGE OF TESTING/ MU
NO. PRODUCTS, TESTS PERFORMED METHOD) OR TECHNIQUE USED LIMIT OF DETECTION (±)
Antilog MATERIALS OR
ITEMS TESTED
1 WATER E. coli/250ml IS 15185 : 2002 Absent/Present NA
(PACKAGED Coliforms/250ml NA
DRINKING
WATER Faecal streptococci/250ml IS 5887 (part2): 1976 NA
OTHER 0.3MPN
THAN Staphylococcus Absent/Present NA
NATURAL aureus/250ml
MINERAL Pseudomonas IS: 13428 ANNEX D.1998 NA
WATER aeruginosa /250ml
IS Sulphite reducing bacteria IS:13428 ANNEX C.1998 Absent/Present NA
14543:2004 /50ml
PACKAGED Aerobic microbial IS 5402 :2002 1 cfu/ml 0.0742
NATURAL 0.0299 count/ml at 22? C & 37?C
MINERAL Yeast & Mould/250ml IS: 5403.1999 Absent/Present NA
WATER IS Salmonella/250ml IS 15187 (part 3):1999 NA
13428:1998 Shigella/250ml IS 5887 ( part 7):1999 NA
DRINKING Vibrio cholerae/250ml IS: 5887 (PART V) NA
WATER Vibrio IS 5887 (part 5) :1976 Absent/Present NA
IS parahaemolyticus/250ml
10500:1991

PROCESS Coliform bacteria IS 1622: 1981: reaffirmed MPN =1/100ml NA
WATER in 1996
IS Standard plate count 1 cfu/ml 0.0299
4251:1967 in 1996
98/83/EC Proteolytic bacteria IS 4251: 1967 Appendix A 5 cfu/ml NA
Lipolytic bacteria 5 cfu/ml NA
stE. coli/100 ml APHA:21 Ed 9221C, F 1 cfu 0.044
MPN =1.8 - 1600
Page 1 of 11 NABL 2009 INTERFIELD LABORATORIES, COCHIN-682005

GROUP OF SPECIFIC TESTS OR TYPES OF SPECIFICATION, SL RANGE OF TESTING/ MU
PRODUCTS, TESTS PERFORMED STANDARD ( METHOD) OR NO. LIMIT OF DETECTION (±)Antilog
MATERIALS TECHNIQUE USED
OR ITEMS
TESTED
1 WATER Faecal streptococci APHA:21st Ed 9230B MPN <1.8 - 1600/100ml NA
Salmonella APHA: 21st Ed 9260B Absent/Present NA
Shigella d 9260E NA
Vibrio cholerae/100 ml d 9260H NA
Pseudomonas aeruginosa APHA: 21st Ed 9213F MPN=1.8 to 1600/100 ml NA
EN 12780:2002 Absent/Present in 250 ml
Heterotrophic plate count d 9215B 1cfu/ml or 1 cfu/100 ml NA
Total coliforms/100 ml APHA: 21st Ed 9221B MPN =1.8 to 1600 NA
Faecal coliforms/100ml d 9221E =1.8 NA
Total Plate Count ISO 6222(1999e) 1 cfu 0.0742
0 0at 22 C & 37 C/ml 0.0299
Enterococci/100 ml IS 15186: 2002 Absent / Present NA
Clostridium perfringens Annex III 98/83 EC Absent /Present NA
including spores/100 ml

Page 2 of 11 NABL 2009 INTERFIELD LABORATORIES, COCHIN-682005
GROUP OF SPECIFIC TESTS OR SPECIFICATION, STANDARD ( METHOD) OR SL RANGE OF TESTING/ MU
PRODUCTS, TYPES OF TESTS TECHNIQUE USED NO. LIMIT OF DETECTION (±)
MATERIALS PERFORMED Antilog
OR ITEMS
TESTED
TH2. MARINE Total plate count/G IS : 5402 – 2002, BAM 8 Ed Ch 3 Jan = 10 cfu per g 0.03
PRODUCTS 2001, AOAC Petrifilm method
RAW Total coliforms/G BAM 8th Ed Ch-4 Sep 2002 IS 5401 = 10 cfu 0.0626
/FROZEN Part 1 & 2, 2002, Petrifilm method MPN =3 to 1100
SQUID =0.3
CUTTLEFISH E. coli/G BAM 8th Ed Ch-4 Sep 2002 – IS 5887 = 10 cfu 0.0905
OCTOPUS (Part 1) 1976, Reaffirmed 1995, AOAC MPN =3 to 1100
FISH Petrifilm method =0.3 to 110
SHRIMPS Staphylococcus BAM 8th Ed Ch-12 Jan 2001, AOAC = 10 cfu 0.028
SQUID aureus/G Petrifilm Method, IS: 5887 (PART II) MPN =3 to 1100
TUBES 1976 =0.3 to 110
TENTACLES Faecal IS: 5887 (PART II) 1976 10 cfu 0.398
AND CRAB streptococci/G MPN =0.3 to 110
MEAT Clostridium BAM 8th Ed Ch-16 Jan 2001 = 10 cfu per g PENDING
perfringens
Salmonella BAM 8th Ed Ch-5 Apr 2003, IS 5887( Absent/Present in 25g NA
Part 3 ) 1999,
Shigella BAM 8th Ed Ch - 6 Jan 2001, NA
IS 5887 ( Part 7 ) 1999
Vibrio cholerae BAM:8th Ed Ch – 9 May 2004 IS 5887 ( NA
Part 5 ) 1976 Reaff 1995
Vibrio BAM 8th Ed Ch-9 May 2004 Absent/Present in 25g NA
parahaemolyticus
Vibrio vulnificus d Ch- NA
Bacillus cereus/G BAM 8th Ed Ch-14 Jan 2001 = 10 cfu 0.0108
MPN =3 to >1100
Listeria d Ch-10 Jan 2003 Absent/Present in 25g NA
monocytogenes Petrifilm Method, ISO 11290-2 : 1998 = 10 cfu per g
thThermo tolerant BAM 8 Ed Ch –4 , Sep 2002 NA
coliforms/ F. MPN = 3 – 1100/g
coliforms
Enterobacteriacea IS 7402:1993, Petrifilm Method = 10 cfu 0.22
MPN = 0.3 – 110/g
thYeast & Mould/G IS: 5403.1999 BAM 8 Ed Ch 18, AOAC 10 cfu NA
Petrifilm Method 100 cfu
Page 3 of 11 NABL 2009 INTERFIELD LABORATORIES, COCHIN-682005


GROUP OF SPECIFIC TESTS OR SPECIFICATION, STANDARD ( RANGE OF TESTING/ MU
PRODUCTS, TYPES OF TESTS METHOD) OR TECHNIQUE USED SL LIMIT OF DETECTION (±)Antilog
MATERIALS OR PERFORMED NO.
ITEMS TESTED
TH3. RAW/ Total plate count IS : 5402 – 2002, BAM 8 = 10cfu 0.03
FROZEN /G Ed Ch – 3 Jan 2001 AOAC
MEAT Petrifilm method
0.0626 PRODUCTS Total coliforms/G BAM 8th Ed Ch- 4 = 10 cfu
COOKED Sept: 2002, IS 5401 Part MPN =3 to 1100
SHRIMPS 1&2: 2002, AOAC Petrifilm =0.3 to 110 method
SQUID 0.0905 E. coli/G BAM 8th Ed Ch - 4 =10 cfu
RINGS Sept: 2002 IS 5887 MPN =3 to 1100
& (Part 1 ) 1976, Reaffirmed =0.3 to 110
TENTACLES 1995, AOAC Petrifilm
method
thThermo tolerant BAM 8 Ed Ch –4 , Sep : NA
coliforms /G 2002 MPN =3 to 1100
thF. coliforms BAM 8 Ed Ch –4 , Sep : NA
2002 MPN =3 to 1100
0.22 Enterobacteriacea IS 7402:1993, AOAC Perifilm =10 cfu
Method
thYeast & IS: 5403.1999, BAM 8 Ed =10 cfu, PENDING
Mould/G Ch 18, AOAC Petrifilm 100 cfu
Method
0.028 Staphylococcus BAM 8th Ed Ch - 12 =10 cfu per g
aureus /G Jan:2001, AOAC Petrifilm MPN <3 to 1100
Method
Clostridium BAM 8th Ed Ch-16 Jan 2001 =10 cfu PENDING
perfringens/G
0.398 Faecal IS: 5887 (PART II) 1976 =10 cfu
streptococci/G MPN =0.3 TO 110
Salmonella BAM 8th Ed Ch - 5 Apr Absent/Present in NA
2003 25g
Page 4 of 11 NABL 2009 INTERFIELD LABORATORIES, COCHIN-682005

GROUP OF SPECIFIC TESTS OR SPECIFICATION, SL RANGE OF TESTING/ MU
PRODUCTS, TYPES OF TESTS STANDARD ( METHOD) NO. LIMIT OF DETECTION (±)Antilog
MATERIALS OR ITEMS PERFORMED OR TECHNIQUE USED
TESTED
Shigella BAM 8th Ed Ch - 6 Absent/Present in 25g NA
Jan: 2001
Vibrio cholerae BAM 8th Ed Ch-9 NA
May 2004
Vibrio BAM 8th Ed Ch-9 NA
parahaemolyticus
Vibrio vulnificus BAM 8th Ed Ch-9 Absent/Present in 25g NA
May 2004
Bacillus cereus/G BAM 8th Ed Ch- =10 cfu .0108
14 Jan 2001 MPN =3 to 1100

Listeria BAM 8th Ed Ch-10 Absent/Present in 25g NA
monocytogenes Jan 2003, Petrifilm =10 cfu per g
Method
ISO 11290-2 :1998
Page 5 of 11 NABL 2009 INTERFIELD LABORATORIES, COCHIN-682005

GROUP OF SPECIFIC TESTS OR SPECIFICATION, STANDARD ( SL RANGE OF TESTING/ MU
PRODUCTS, TYPES OF TESTS METHOD) OR TECHNIQUE USED NO. LIMIT OF DETECTION (±)Antilog
MATERIALS OR PERFORMED
ITEMS TESTED
TH4. BLACK Total plate count IS : 5402, 2002, BAM 8 Ed = 10cfu per g 0.477
PEPPER, /G Ch– 3 Jan 2001 AOAC
PROCESSED Petrifilm method
FOOD Enterobacteriaceae IS 7402:1993, AOAC Petrifilm = 10cfu 1.274
CONTAINING /G Method MPN =0.3 to 110
thRED PEPPER Yeast & Mould/G IS: 5403.1999 BAM 8 Ed Ch =10 cfu, =100 cfu 1.017
AND OTHER 18, AOAC Petrifilm Method
SPICES AND Total coliforms/G BAM 8th Ed Ch-4 Sep 2002, = 10 cfu per g 1.274
CONDIMENTS AOAC Petrifilm Method MPN <3 to 1100
E. coli/G d Ch-4 Sep 2002, =10 cfu 1.274
MPN =3 to 1100
Staphylococcus BAM 8th Ed Ch-12 Jan =10 cfu PENDING
aureus /G 2001, AOAC Petrifilm Method MPN =3 to 1100
Clostridium BAM 8th Ed Ch-16 Jan:2001 =10 cfu per g PENDING
perfringens
Faecal IS: 5887 (PART II) 1976 =10cfu
streptococci/G =0.3 MPN to 1100

Salmonella BAM 8TH Ed.Ch- 5 April Absent/Present in 25g NA
2003
Shigella BAM 8th Ed Ch-6 Jan: 2001 NA
Bacillus cereus d Ch-10 Jan 2003 NA

thListeria BAM 8 Ed Ch-14 Jan 2001, Absent/Present in 25g, NA
monocytogenes AOAC Petrifilm Method =10 cfu/g
ISO 11290-2 : 1998
Lactic acid bacteria IS 12899:1989 =10 cfu per g PENDING



Page 6 of 11 NABL 2009 INTERFIELD LABORATORIES, COCHIN-682005
GROUP OF SPECIFIC TESTS OR TYPES SPECIFICATION, STANDARD ( SL RANGE OF TESTING/ MU
PRODUCTS, OF TESTS PERFORMED METHOD) OR TECHNIQUE USED NO. LIMIT OF DETECTION (±)Antilog
MATERIALS OR
ITEMS TESTED
TH5 MILK & MILK Total plate count/G IS : 5402, 2002, BAM 8 =10 cfu 0.266
POWDER Ed Ch–3 Jan 2001 AOAC
Petrifilm method
PENDING Total coliforms/G IS 5401 ( Part 1) 2002, IS =10 cfu
5401 Part 1 2002, BAM MPN =3 to 1100
th8 Ed Ch 4 Sep 2002, =0.3 to 110
AOAC Petrifilm Method
th PENDING Yeast & Mould/G IS: 5403.1999, BAM 8 =10 cfu
Ed Ch 18, AOAC Petrifilm =100 cfu
Method
PENDING E. coli/G IS 5887 ( Part 1) 1976 =10 cfu
Reaffirmed 1995, BAM MPN =3 to 1100
8th Ed Ch-4 Sep 2002, =0.3 to 110
AOAC Petrifilm Method
PENDING Faecal streptococci/G IS 5887 (part2) 1976 =10 cfu
MPN =0.3 to 110
PENDING Staphylococcus aureus IS 5887 (part2) 1976, =10 cfu
/G BAM 8th Ed Ch-12 MPN =3 to >1100
Jan2001, AOAC Petrifilm =0.3 to 110
Method
NA Shigella IS 5887 (part7)1999, BAM Absent/Present in 25g
8th Ed Ch-6 Jan 2001,
Petrifilm Method
NA Salmonella IS 5887 (part3):1993,
BAM 8TH Ed.Ch- 5 Apr
2003, Petrifilm Method
PENDING Enterobacteriacea/G IS 7402:1993 , Petrifilm =10 cfu
Method
NA Bacillus cereus BAM 8th Ed Ch-14 Jan Absent/Present in 25g
2001
NA Listeria monocytogenes -10 Jan
2003 Absent/Present or
Petrifilm Method =10 cfu per g or ml Page 7 of 11
ISO 11290-2 : 1998 NABL 2009 INTERFIELD LABORATORIES, COCHIN-682005



SL GROUP OF SPECIFIC TESTS OR SPECIFICATION, STANDARD ( METHOD) OR RANGE OF TESTING/ MU
PRODUCTS, TYPES OF TESTS TECHNIQUE USED NO. LIMIT OF DETECTION (±)
MATERIALS OR PERFORMED
ITEMS TESTED
TH6. Starch Total plate count /G IS : 5402, 2002, BAM 8 Ed Ch – =10 cfu Pending
(Maize 3 Jan 2001, AOAC Petrifilm method
thStarch, Total coliforms/G IS 5401 ( Part 1) : 2002, BAM 8 =10 cfu Pending
Arrow root Ed Ch 4 Sep 2002, AOAC Petrifilm MPN =3 to 1100
starch, Method =0.3 to 110
thtapioca Yeast & Mould/G IS: 5403, 1999, BAM 8 Ed Ch 4 =10 cfu Pending
starch Sep 2002, AOAC Petrifilm Method =100 cfu
Corn flour) E. coli/G IS 5887 ( Part 1) 1976 Reaffirmed =10 cfu Pending
th 1995, BAM 8 Ed Ch 4 Sep 2002, MPN =3 to 1100
AOAC Petrifilm Method =0.3 to 110
Faecal IS 5887 (part2) 1976 =10 cfu Pending
streptococci/G MPN
thStaphylococcus IS 5887 (part2) 1976, BAM 8 Ed =10 cfu Pending
aureus /G Ch 12 Jan 2001, AOAC Petrifilm MPN =3 to >1100
Method =0.3 to 110
Clostridium BAM 8th Ed Ch-16 Jan 2001 =10 cfu Pending
perfringens
thSalmonella IS 5887 (part3):1993, BAM 8 Ed Absent/Present in NA
Ch 5 Apr 2003 25g
thShigella IS 5887 (part7)1999, BAM 8 Ed NA
Ch 6 Jan 2001 25g
Bacillus cereus BAM 8th Ed Ch-14 Jan :2001 =10 cfu per g, Pending
<3MPN to 1100
Enterobacteriacea/G IS 7402:1993, AOAC Petrifilm =10 cfu Pending
Method

Page 8 of 11 NABL 2009 INTERFIELD LABORATORIES, COCHIN-682005
GROUP OF SPECIFIC TESTS OR TYPES SPECIFICATION, STANDARD SL RANGE OF TESTING/ MU
PRODUCTS, OF TESTS PERFORMED ( METHOD) OR TECHNIQUE USED NO. LIMIT OF DETECTION (±)
MATERIALS
OR ITEMS
TESTED
th =10 cfu NA 7. Cooked Total plate count /G IS : 5402, 2002, BAM 8 Ed
food stuffs Ch 3, AOAC Petrifilm Method
Ready to
eat =10 cfu NA Total coliforms/G IS 5401 ( Part 1) : 2002, BAM
th8 Ed Ch 4 Sep 2002 AOAC MPN =3 to 1100 processed =0.3 to 110 Petrifilm Method food stuffs
th =10 cfu NA Yeast & Mould/G IS: 5403, 1999, BAM 8 Ed Ch
=100 cfu 18, AOAC Petrifilm Method
=10 cfu NA E. coli/G IS 5887 ( Part 1) 1976
th MPN =3 to 1100 Reaffirmed 1995, BAM 8 Ed =0.3 to 110 Ch 4 Sep 2002, AOAC Petrifilm
Method
=10 cfu NA Faecal streptococci/G IS 5887 (part2) 1976
MPN =0.3 to 110
thStaphylococcus aureus IS 5887 (part2) 1976, BAM 8 =10 cfu NA
MPN =3 to 1100 Ed Ch 12 Jan 2001 AOAC /G
Petrifilm Method
=10 cfu NA Clostridium perfringens BAM 8th Ed Ch - 16 Jan 2001
th NA Salmonella IS 5887 (part3):1993, BAM 8 Absent/Present in 25g
Ed Ch 5 Apr 2003
th NA Shigella IS 5887 (part7)1999, BAM 8 Absent/Present in 25g
Ed Ch 9 May 2004
=10 cfu NA Bacillus cereus/G BAM 8th Ed Ch-14 Jan 2001
MPN =3 to 1100

IS 7402: 1993, Petrifilm Method =10 cfu NA Enterbacteriacea/G
thBAM 8 Ed Ch 4 Sep 2002 NA Thermo tolerant MPN =3 to 1100
Coliforms/Faecal per G



Page 9 of 11 NABL 2009 INTERFIELD LABORATORIES, COCHIN-682005

GROUP OF PRODUCTS, SPECIFIC TESTS OR TYPES OF SPECIFICATION, STANDARD RANGE OF MU
MATERIALS OR ITEMS TESTS PERFORMED ( METHOD) OR TECHNIQUE TESTING/ (±) SL
TESTED USED LIMIT OF NO.
DETECTION
8. Shrimps, Squid and Antibiotic Residues
aquatic products pending Chloramphenicol Enzyme immunoassay for 100 to
the quantitative analysis 1000ppt/100ppt
of Chloramphenicol Art.
No. R1501, R-Biopharm
AG, Damstadt, Germany
pending Sulfamethazine 100 to 1000ppt/2ppb
of Sulfamethazine Art. No.
R3001, R-Biopharm AG,
Damstadt, Germany
Nitrofuran(AOZ) Enzyme immunoassay for 200 to pending
the quantitative analysis 1000ppt/200ppt
of AOZ Art. No. R3701, R-
Biopharm AG, Damstadt,
Germany
pending Tetracycline Enzyme immunoassay for 6 to 500ppb/6 ppb
Oxytetracycline of Tetracycline Art. No. 60 to pending
R3501, R-Biopharm AG, 500ppb/60ppb
Damstadt, Germany



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